lenticrisprv2 opti puro (Addgene inc)
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lenticrisprv2 opti puro
Lenticrisprv2 Opti Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+opti/lentiCRISPRv2+puro+(Plasmid+%2398290)/bio_rxiv__64898__2026__02__01__703113-230-14-16
Average 98 stars, based on 517 article reviews
Lenticrisprv2 Opti Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+opti/lentiCRISPRv2+puro+(Plasmid+%2398290)/bio_rxiv__64898__2026__02__01__703113-230-14-16
Average 98 stars, based on 517 article reviews
lenticrisprv2 opti puro - by Bioz Stars,
2026-09
98/100 stars
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Plasmid Preparation:Article Title: Genome-scale CRISPR screening in a single mouse liver Article Snippet: The pLentiCRISPRv2-Stuffer-HepmCherry and pLentiCRISPRv2-Stuffer-HepmTurquoise2 vectors were produced through the following steps: 1) removal of the EFS-NS promoter and Cas9 from the parental vector and insertion of a hepatocyte-specific promoter driving dsRed expression, 2) replacement of dsRed with mCherry or mTurquoise2, and 3) removal of the puromycin resistance cassette. .. To produce pLentiCRISPRv2-Stuffer-HepdsRed-Puro, 100 ng of a synthetic gblock encoding the HS-CRM8-TTRmin module upstream of dsRed (Integrated DNA Technologies) and 1 μg of Article Title: The integrated stress response fine-tunes stem cell fate decisions in response to serine deprivation and tissue injury Article Snippet: For qPCR equal amounts of cDNA template were mixed with validated gene-specific primers, nuclease-free water and SYBR green PCR Master Mix (ThermoFisher). qPCR was performed with technical replicates using an Applied Biosystems 7900HT Fast Real-Time PCR machine and relative expression was calculated as RE = 2 −ΔΔCt . .. Five sgRNAs per gene were picked using the VBC-score tool 108 , and oligos with overhangs compatible for cloning into the Cloning:Article Title: The integrated stress response fine-tunes stem cell fate decisions in response to serine deprivation and tissue injury Article Snippet: For qPCR equal amounts of cDNA template were mixed with validated gene-specific primers, nuclease-free water and SYBR green PCR Master Mix (ThermoFisher). qPCR was performed with technical replicates using an Applied Biosystems 7900HT Fast Real-Time PCR machine and relative expression was calculated as RE = 2 −ΔΔCt . .. Five sgRNAs per gene were picked using the VBC-score tool 108 , and oligos with overhangs compatible for cloning into the Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with Synthesized:Article Title: The integrated stress response fine-tunes stem cell fate decisions in response to serine deprivation and tissue injury Article Snippet: For qPCR equal amounts of cDNA template were mixed with validated gene-specific primers, nuclease-free water and SYBR green PCR Master Mix (ThermoFisher). qPCR was performed with technical replicates using an Applied Biosystems 7900HT Fast Real-Time PCR machine and relative expression was calculated as RE = 2 −ΔΔCt . .. Five sgRNAs per gene were picked using the VBC-score tool 108 , and oligos with overhangs compatible for cloning into the Clone Assay:Article Title: Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction. Article Snippet: The raptor antibody was from EMD Millipore (09-217); S6K1 pT389 (9234), S6K1 (2708), rpS6 pS235/S236 (2211), rpS6 (2217), Sestrin-2 (8487), ATF-4 (11815), raptor pS792 (2083), acetyl-CoA carboxylase (ACC) (3662), ACC pS79 (3661), AMPKα (5831), AMPKα pT172 (2535), and GCN2 (3302) antibodies were from Cell Signaling Technology; ND1 (19703-1-AP), Sestrin2 (10795-1-AP), and Redd1 (10638-1-AP) antibodies were from Proteintech; HRI (MBS2538144) antibody was from MyBioSource; and the HSD17B10 (TA500724) antibody was from Life Technologies. sgRNA Cloning, Lentiviral Production, and Lentiviral Transduction. .. Individual sgRNAs (SI Appendix, Dataset S3) were cloned into Generated:Article Title: DMXL1 promotes recruitment of V1-ATPase to lysosomes upon TRPML1 activation. Article Snippet: .. U2OS-KO cells were generated by introducing Cas9 and single guide RNA (sgRNA) through lentiviral particles packaged using Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with other:Article Title: The integrated stress response fine-tunes stem cell fate decisions upon serine deprivation and tissue injury. Article Snippet: The following primary antibodies and dilutions were used: PHGDH (rabbit, 1:1,000, Sigma); ATF4 (rabbit, 1:1000, Cell Signaling), phospho-eIF2⍺ (rabbit, 1:1000, Cell Signaling), total eIF2⍺ (rabbit, 1:5,000, Cell Signaling), ASNS (1:2000; Proteintech); phosphoPERK (T980) (rabbit, 1:1000, Cell Signaling), total PERK (rabbit, 1:500, Cell Signaling), phospho-GCN2 (T899) (rabbit, 1:500, Abcam), total GCN2 (rabbit, 1:1000, Cell Signaling), PSAT1 (mouse, 1:500, Abnova), PSAT1 (rabbit, 1:1000, Proteintech), PSPH (rabbit, 1:500, Sigma), CAS9 (mouse, 1:1000, Active Motif), H3K27me3 (rabbit, 1:1000, Cell Signaling), H3 (mouse, 1:5000, Cell Signaling), and VCL (mouse, 1:5000, Sigma). Knock-Out:Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with CRISPR:Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with Infection:Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with Ligation:Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with Isolation:Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with Fluorescence:Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with FACS:Article Title: Solute carrier family 46 member 3 (SLC46A3) as marker for lipid-based nanoparticle cancer therapy and diagnostics Article Snippet: .. SLC46A3 Knock-Out Via CRISPR-Cas9 in T47D Cells SLC46A3 knock-out T47D cell lines were generated by infection with |